A mixture of proteases, amylases, cellulases, and lipases. The primary use is in septic treatment products or bioremediation formulas. It is generally used as part of a blend containing enzymes, nutrients, and microorganisms.

By definition, an ENZYME is a protein molecule found in living cells which initiates a chemical process.
ENZYMATIC DEVELOPERS utilize proteolytic enzymes which are enzymes built from Amino Acids. The diluted proteins that Enzyme developers contain in their formulation are so small they can actually strip imperfections on the cuticle layers, which improves the

 Introduction Enzymes are used today in a varied and growing number of industries – from animal feed to alcohol production; from dairy products to detergents; from tenderizing to textiles. As the number of applications continues to grow and become specialized, the selection of the appropriate enzyme becomes critical.
There are numerous enzymes available from many suppliers, further complicating the selection process. The most important thing to remember is that enzymes do vary from type to type, and from supplier to supplier. However, with a basic understanding of these differences, the selection process becomes much easier. This brochure is written as a basic guide, to facilitate the purchasing selection process.
What are Enzymes? Enzymes are proteins, which act as a catalyst in many reactions. They are present in all animals, organisms, and some plants, to help break down various foods into components that are readily utilized. In nature, enzymes only catalyze a single type of reaction; therefore, their commercial use is also very specific.
Enzymes are not living organisms, although they are derived from living organisms (including plants and animals). It is through the careful isolation and extraction of these enzymes that commercially available products are available to the market.
Important Buying Criteria:
 Often times, the research department or the marketing department will not give the purchasing agent the information needed to make wise buying choices. The PA may be told, “We need a price for X enzyme”. There is not enough information! This is no different than telling the PA to “go buy a horse” or, to “go buy a car”. There is not enough information to know what to buy. The buyer could buy a racehorse or a broken-down nag. The buyer could buy a Porsche or a rust bucket. In both cases, the PA has done what was requested. There are many important criteria to be considered when selecting or purchasing enzymes. An understanding of these criteria is essential to help select the appropriate enzyme, but also to help the buyer compare costs of enzymes from various suppliers.
Category of Enzyme Enzymes are categorized by the reaction they catalyze. Most of the food enzymes ENZECO® LYSOZYME CHLORIDE
A powder or liquid preparation derived from egg white. It has the ability to lyse cell wall of various microorganisms such as lactobacillus and certain detrimental yeasts in the wineries. The traditional application has been to prevent “gas formers ” in cheese production but it has expanded to many other applications susceptible to lactobacillus infection.

Hydrolyzes residual urea in wines to prevent conversion of the urea and ethanol to ethyl carbamate. Used primarily in wines that are baked or otherwise exposed to heat for an extended time. It is also useful in wine when urea levels exceed 5 ppm.

An enzyme preparation specific to the breakdown of hydrogen peroxide. It is available in textile grade and in food grade. It is not available as a kosher certified product.


hairs surface. Enzymes are made from Plant proteins which attract to other proteins, in this case keratin. Its like a magnet.  There is no need to lift the cuticle for the color to attach and therefor there is no fading and the hair isn’t compromised in any way.   My clients with porosity and fading issues have had amazing results.  This is the answer to healthy colored hair, why is this amazing product not used by most hairdressers?  Has any one had any experience with Enzymes?

A process is disclosed for conversion of monosaccharides, disaccharides and trisaccharides into aldonic acids by means of dehydrogenase enzyme containing cells without a hydrogen acceptor and without the nutrients and conditions necessary for cell growth. High purity maltobionic and lactobionic acids may be formed thereby during the manufacturing process of the disaccharide. Glucose, maltose and maltotriose in starch syrups of varying compositions may also be so converted to their corresponding aldonic acids.

An enzyme-based oxidative process for coloring hair wherein the hair is exposed to a solution having a pH of about 4 to about 10 and containing hydrogen peroxide, soybean peroxidase enzyme and one or more oxidation dye precursors.


Chemicals to avoid-
Propylene Glycol
A cosmetic form of mineral oil found in automatic brake and hydraulic fluid, and industrial antifreeze. In skin and hair care products propylene glycol works as a HUMECTANT, which is a substance that retains the moisture content of skin or cosmetic products by preventing the escape of moisture or water. Material Safety Data Sheets warn users to avoid skin contact as this strong skin irritant can cause liver abnormalities and kidney damage.

Sodium Lauryl (laurel) Sulfate

SLS is well known in the scientific community as a common skin irritant. It is rapidly absorbed and retained in the eyes, brain, heart, and liver, which may result in harmful long-term effects. SLS could retard healing, cause cataracts in adults and keep children’s eyes from developing properly.

Sodium Laureth Sulfate

SLES is the alcohol form (ethoxylated) form of SLS. It is slightly less irritating that SLS, but may cause more drying. Both SLS and SLES may cause potentially carcinogenic formations of nitrates and dioxins to form in shampoos and cleansers by reacting with an other ingredients.

Diethanolamine (DEA)

A colorless liquid or crystalline alcohol that is used as a solvent, emulsifier, and detergent (wetting agent). DEA works as an emollient in skin softening lotions or as a humectant in other personal care products. When found in products containing nitrates, it reacts chemically with the nitrates to form potentially carcinogenic nitrosamines. DEA may also irritate the skin and mucous membranes. It is known that we should avoid using other ethanolamines,which may also be snown as triethanolamines, (TEA), and also monoethanolamine (MEA)

Methyl, Propyl, Butyl & Ethyl Parabens

Parabens are used as inhibitors of microbial growth and to extend shelf life of products. Widely used even though they are known to be toxic. Parabene have caused many allergic reactions and skin rashes. Methyl paraben combines benzoic acid with the methyl group of chemicals. Highly toxic.

This invention relates to an enzyme activated process for coloring hair.

Pre-formed dyes for coloring hair have not gained wide acceptance due to their general failure to impart colors to hair which are sufficiently imitative of natural hair colors. Products which contain reactants such as H2 O2 and an oxidation dye precursor (i.e., a precursor which forms dyes in situ on the hair through an oxidative process) do produce colors which are closely imitative of natural hair color and have obtained reasonable commercial success. These latter products, however, because of the severe oxidizing conditions required (i.e., performing the oxidation at H2 O2 concentration of 3% or more and a pH of 8.5 or higher for time periods in excess of 20 minutes) and the high concentrations of oxidation dye precursors needed to produce the desired coloration can cause skin irritation and sensitizatin as well as hair damage to some users. Further, the ammonium hydroxide which is generally used to maintain the high pH in these products has an odor which is offensive to most users.


The object of the invention herein is to provide an improved mild oxidative process for coloring hair.

This and other objects, which will become apparent, are achieved by the invention herein which is an enzyme-activated oxidative process for coloring hair wherein hydrogen peroxide is used as the oxidizing agent. The enzyme used in the process herein is soybean peroxidase and the process comprises contacting the hair with a solution comprising soybean peroxidase enzyme, hydrogen peroxide and one or more oxidation dye precursors (as hereinafter defined), said solution having a pH of from about 4.0 to about 10.0, preferably from about 5.5 to about 8.0.

Soybean peroxidase is a member of the peroxidase enzyme family classified in Class 1.11 [i.e., Class 1, Subclass 11 of the Recommendation (1964) of the International Union of Biochemistry on the Nomenclature and Classification of Enzymes], which catalyze the oxidation of various materials (including the oxidation dye precursors herein) by hydrogen peroxide. Soybean peroxides is, as its name implies, derived by known methods from the soybean hull.

The soybean peroxidase enzyme can be used in its pure crystalline form, which is obtained by isolating the enzyme from other materials present during preparation, or it can be used in a diluted form where the enzyme is present in a composition along with these materials and/or added inert diluents.

Commercially available enzyme preparations normally contain the enzyme in combination with inert diluent and carrier materials such as carbohydrates, agglutinating proteins, inorganic salts such as sodium sulfate, calcium sulfate, and the like. In such preparations the enzyme constitutes a minor component and comprises from about 1% to about 50% by weight of the preparation. The remaining 50% to 99% is comprised of the hereinbefore described diluents and carriers. The commercially available enzyme-containing preparations are preferred as sources of enzyme herein as they are more readily available than the pure crystalline enzyme and provide known, pre-determined and desirable levels of enzyme activity.

In the coloring process herein, soybean peroxidase enzyme is used at concentrations of from about 0.01 ppm to about 500 ppm, and preferably from about 0.05 ppm to about 100 ppm in the coloring solution. These levels are based on weight of pure enzyme. If a commercial enzyme preparation is used wherein the enzyme is combined with diluents and carriers, as hereinbefore described, the concentration of the enzyme preparation will have to be proportionately higher in order to achieve the required concentration of pure enzyme. The amount of pure enzyme present in such compositions can be readily determined by known assay methods.

The oxidation dye precursors which are used in the compositions and processes herein include aromatic diamines, various substituted phenols, amino phenols and derivatives of these aromatic compounds (e.g., N-substituted derivatives of the amines and ethers of the phenols). The oxidation dye precursors useful herein can be classified as “primary oxidation dye precursors” and “secondary oxidation dye precursors”, as detailed hereinafter. In general terms, oxidation hair dye precursors include those monomeric aromatic compounds which, on oxidation, form aligomers or polymers having extended conjugated systems of electrons in their molecular structure. Because of the new electronic structure, the resultant oligomers and polymers exhibit a shift in their electronic spectra to the visible range and appear colored. For example, oxidation dye precursors capable of forming colored polymers include materials such as various aromatic amines having a single functional group and which, on oxidation, form a series of conjugated imines and quinonoid dimers, trimers, etc. ranging in color from green to black. Compounds, such as p-phenylenediamine, which have two functional groups are capable of oxidative polymerization to yield higher molecular weight colored materials having extended conjugated electron systems, i.e., the so-called “Bandrowski’s Base” type of dye compound. Color modifiers, such as those detailed hereinafter as “secondary oxidation dye precursors”, can optionally be used in conjunction with the primary oxidation dye precursors herein and are thought to interpose themselves in the colored polymers during their formation and to cause shifts in the electronic spectra thereof, thereby resulting in changes in color and/or color intensity. It is to be understood that the peroxidase enzymes disclosed herein are suitable for use (in conjunction with a peroxide source, e.g., H2 O2, as detailed herein) with all manner of primary and secondary oxidation dye precursors.

They have different developers available: liquid, cream, and a liquid enzyme developer that is water, hydrogen peroxide, water soluble polysaccharides, and polymers that range from 5 volume to 40 volume.  According to the class that I took, this enzyme developer does not activate until you apply heat, and it can process the color in 1/2 the time.  (I don’t use it in this way, but find it is good for resistant gray coverage).  They do have a NN series as well, which does not contain double pigment, but rather more oil to help soften the hair more.  There is also an ammonia-free lightener that uses potassium persulfate that gives up to 7 levels of lift.  It can be used on or off the scalp.



Hair color is made up of two components: color and developer. The developer contains hydrogen peroxide and ammonia to modify the molecular structure of the hair shaft, allowing large color molecules to penetrate while phenylenediamine (PPD) allows the color to bond with the hair. It is now known that some permanent hair dyes may also contain coal tar, a potentially detrimental petrochemical, and toxic metals such as lead or mercury as developers.


By using Enzymes your hair will shift away from unwanted brassy tones. Peroxide turns orange as soon as it touches the hair.  Do you remember Sun In? Everyone who used Sun in had bright orange or gold hair. Enzymes lift and deposit beyond the orange stage with out damaging the hair or it’s unwanted brassy tones, leaving the hair in better condition.

Researchers at The University of California discovered that individuals coloring their hair with permanent dyes once a month for a year or more have twice the risk of developing bladder cancer. Hairdressers or barbers in contact with hair dyes had five times the risk.  We are against fear mongering, but we fully are dedicated to knowledge and better choices. 

What’s Next: Our New Love!

We’ve seen incredible results with our recent switch. Enzyme developers are naturally formed proteins that neutralize the hair and leave it in superior condition. Continued use of this product, combined with our naturally zero ammonia color and home care products, will restore your hair to its original shine and luster.

You probably have a lot of questions: What is it? What does it do? Well, up until now, your traditional developer used hydrogen peroxide. Both alkaline agents and hydrogen peroxide swell the cuticle so that the color pigments can penetrate into the cortex.  When applied inappropriately, the hair can burst and become damaged over time.  For this reason, we’v shifted to enzymes (alkaline) to efficiently and gently open and close the cuticle that results in longer lasting color and healthier hair. 

Why doesn’t every salon divorce hydrogen peroxide?

It’s all about the money, honey! Our ethos is to keep you and your follicles healthy. That’s how we get real results. Yes, this does raise our overall costs for color a smidge, but aligned with our mission and big picture view of health and beauty – it’s worth it!  We hope you see it this way, too.

If you prefer to use hydrogen peroxide, please let your stylist know and we will revert back to old systems for your needs.  Hair color pricing is variable depending on the stylist and color amount used.  Enzyme developer use has increased our color charges by only $3.  We trust you’ll see the lasting benefits! 


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